Dhx9 atpase assay

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August undefined, 2024

WebDHX9. On the top, protein expression in current human tissue, based on all annotated cell types, is reported with the units not detected (n), low (l), medium (m) and high (h). … WebImportantly, binding of Nup98 to DHX9 stimulates the ATPase activity of DHX9, and a transcriptional reporter assay suggests Nup98 supports DHX9-stimulated …

Q08211 - UniProt

WebRNA-seq analysis showed that the Dhx9 deficiency caused an upregulated DNA damage response pathway and Gadd45, Cdkn1a, Cdc25, Wee1, and Myt1 expression to induce … WebJun 4, 2013 · Various results, including in vitro assays, show that the FG/GLFG region of Nup98 binds to N- and C-terminal regions of DHX9 in an RNA facilitated manner. Importantly, binding of Nup98 stimulates the ATPase activity of DHX9, and a transcriptional reporter assay suggests Nup98 supports DHX9-stimulated transcription. church street cafe dubbo

Establishment of a Primary Screening Assay for the DHX9 …

WebJun 6, 2024 · The RNA helicase DHX9 associates with IGS-rRNA pRNA is a 250–300 nucleotide transcript that derives from processing of the 2-kb … WebFeb 21, 2024 · We have shown using a combination of in vitro and in vivo assays that Nup98 directly binds DHX9 in the nucleoplasm and this interaction is facilitated by RNA. Importantly, binding of Nup98 to … WebMay 8, 2024 · Because DHX9-depleted cells exhibit a termination defect (Figures 4 H and 4I), we examined the potential role of DHX9 in preventing R-loop-associated DNA damage. For these studies, we used the Top1 inhibitor camptothecin (CPT), which is known to promote R-loop accumulation and replication- and transcription-associated DNA damage … dewy rose post office

High expression of DHX9 promotes the growth and metastasis …

Human Nup98 regulates the localization and activity of …

WebNov 13, 2024 · The proliferation rates of DHX9-overexpressed or -silenced CRC cells were determined by MTS assay and trypan blue exclusion assay. We found that there was no … WebJ Immunol. Author manuscript; available in PMC 2013 May 17. Published in final edited form as: J Immunol. 2011 Nov 1; 187(9): 4501–4508. dewy rose ga to hartwell gaWebJul 23, 2010 · Competition assays were consistent with these findings as all four NTPs inhibited DHX9 ATPase activity (Fig. 1D) [33]. Taken together, these results indicate that our recombinant DHX9 preparation ... church street cafe greensboro nc

"WebMay 8, 2024 · A top candidate DHX9 helicase promotes R-loop suppression and transcriptional termination. DHX9 interacts with PARP1, and both proteins prevent R … " - Dhx9 atpase assay

Dhx9 atpase assay

WebJul 5, 2016 · Initial helicase assays demonstrated double-stranded DNA unwinding activity in bovine DHX9 and RNA unwinding activity with the human orthologue; however, … WebMar 6, 2024 · ATPase Assays. One of the most robust uses of measuring ATPase inhibition is with an ATPase Activity Assay. The protocol developed by BellBrook Labs has been in use for over ten years, with …

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WebJul 23, 2010 · The SAT motif has been shown by mutagenesis to uncouple ATPase activity from RNA helicase activity in both DEAD-box 46, 47, ... NTPase assay. DHX9 325–840 was diluted into an assay buffer [20 mM Hepes (pH 7.5), 3 mM MgCl 2, 300 mM NaCl, 10% glycerol, and 2 mM TCEP] up to final concentrations of 7–80 nM. The assay was … WebNov 1, 2011 · DHX9 is a member of the DExDc helicase family and is a homolog of Drosophila maleless protein, which is essential to regulate X-linked gene expression and the survival of male larvae ().DHX9 …

WebCompare DHX9 Kits from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more. WebSep 17, 2013 · Our ChIP assays indicate that DHX9 was stably bound to the H-DNA-forming region of the c-MYC promoter sequence. Thus, it is likely that the ChIP assays captured specific interactions between DHX9 and H-DNA in chromatin-plasmid complexes in human cells. ... FANCJ uses its motor ATPase to destabilize protein-DNA complexes, …

WebJun 10, 2011 · For competition assays, unlabeled competitor substrates (D-loops, R-loops, forks and G4 structures) were preincubated with DHX9 for 3 min at 25 °C in helicase …

WebCCK-8 assay showed that DHX9 knockdown significantly inhibited the proliferation of MHCC97H cells at 48, 72, and 96 h (Figure 4B). In the scratch assay, DHX9 siRNA-treated cells covered almost 73% of scratches in 24 h and at least 85% in 48 h, while the cells in the NC group covered only 18% in 24 h and 25% in 48 h (Figure 4C,D).

WebAug 1, 2012 · The goal of this project is to develop HTS assays that will allow discovery of small molecule inhibitors of DHX9, which in turn, can be used in cell and animal models to examine the consequences of inhibition of this protein on cancer cell survival. ... Pelletier, Jerry (2015) Establishment of a Primary Screening Assay for the DHX9 Helicase ... church street cafe in old town albuquerqueWebKeywords: ATPase assay, ATA, aurintricarboxylic acid, DHX9, HTS, RNA binding, RNA helicase. Abstract: The RNA helicase DHX9 is an ATP-dependent DExH box helicase that can unwind DNA and RNA. Much evidence has implicated DHX9 at multiple levels of gene expression regulation ranging from genome stability and replication, to transcriptional ... dewy rose georgia countyWebJul 30, 2024 · A co-IP assay was performed to assess the interaction of Flag-tagged WT and methylarginine binding-deficient (E691K) TDRD3 with endogenous DHX9 in MCF7 cells. The input and αFlag antibody-immunoprecipitated samples were analyzed by western blot using indicated antibodies. (D) DHX9 interacts with TDRD3 in an arginine methylation … dewy shampooWebTwo NDD-associated variants, p.(Gly411Glu) and p.(Arg761Gln), altered DHX9 ATPase activity. The severe NDD-associated variant p.(Arg141Gln) did not impact DHX9 localization but instead increased R ... church street car park calneWebAug 1, 2024 · The complex biology of DHX9 was recently reviewed [221]. Helicase and competition assays proved DHX9 to bind and resolve G4s with stronger kinetics toward rG4 and strict ATP-dependence in vitro, as confirmed by the complete loss of G4-unwinding function upon ATPase inhibition by N-ethylmaleimide poisoning [222]. dewy set spray anastasia beverly hillsWebNov 2, 2024 · Much interest has been expended lately in characterizing the association between DExH-Box helicase 9 (DHX9) dysregulation and malignant development, however, the enigmatic nature of DHX9 has … church street car park gatesheadWebOct 19, 2015 · The RNA helicase DHX9 is an ATP-dependent DExH box helicase that can unwind DNA and RNA. Much evidence has implicated DHX9 at multiple levels of gene expression regulation ranging from … church street car park hungerford