Ctsk western blot

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WebAug 3, 2024 · Indeed, when we performed two-dimensional gel electrophoresis followed by Ctsk Western blotting we observed a shift of Ctsk toward a more acidic pH in Acp2/Acp5 –/– osteoclasts (Fig. 1I). … WebApr 13, 2024 · b Western blot analysis of Ano1 protein level in Ano1 fl/fl and Ctsk-Cre;Ano1 fl/fl osteoclasts after treatment with Ctrl or HG (left). The quantification of Ano1 protein level in osteoclasts (right).

Western Blot Technique: Principle, Steps, Uses

WebWestern blot - CTSK Rabbit pAb (A1782) Western blot analysis of extracts of various cell lines, using CTSK antibody (A1782) at 1:500 dilution. Secondary antibody: HRP Goat … WebFeb 12, 2024 · 2.3 各组细胞增殖活性、细胞中trap5b 水平、ctsk 和ctr mrna 表达水平及细胞凋亡率与对照组比较，培养4 d后，rankl组细胞增殖活性明显升高（p＜0.01），细胞中trap5b 水平和ctsk 及ctr mrna 表达水平明显升高（p＜0.01），细胞凋亡率明显降低（p＜0.01）；与rankl 组比较 ... greenline bamboo cups

Mechanical stimulation controls osteoclast function …

WebRabbit polyclonal antibody raised against synthetic peptide of CTSK. IgGy Antibody Selector – Quickly search hundreds of thousands of antibodies available for purchase from VWR by selecting common antibody features like antigen symbol and name, reactivity, clonality, conjugation, host, and other key factors. Antibodies used to identify and locate … WebApr 13, 2024 · Western blotting analysis and monitoring of cell viability. To differentiate the C2C12 cells to myotube, cells were grown in D-MEM medium supplemented with 2% FBS, 1 nM insulin. ... (TRAP), a typical marker protein for osteoclasts, as well as cathepsin K (CTSK), and matrix metalloproteinase-9 (MMP-9) [14, 15]. The TRAP, CTSK, and MMP … WebThe expression of Ctsk was inhibited by adeno-associated virus (AAV). Micro-CT, immunohistochemistry (IHC), Western blot and quantitative real-time polymerase chain … greenline bass clarinet

Western Blotting- Definition, Principle, Steps, Results, Uses

Image Analysis and Quantitation for Western Blotting Bio-Rad

WebJan 16, 2013 · Western blotting analysis showed a reduction of more than 90% in cathepsin K protein levels in CD11b;Ctsk fl/fl osteoclasts (Figure 2F). However, qRT … WebA protein band is a feature that appears in a western blot image. A western blot image is made up of pixels, which contain information about how much signal was collected at each location in the image. A digital image of a blot can be thought of as data in three dimensions. Each pixel in a blot image has an x and y coordinate, in addition to an ... flying fish for saleWebMar 25, 2024 · Western Blot staining of Human Lung lysate using PA5-18950 at a concentration of 1.0 ug/ml, the primary antibody incubation was 1 hour and the detection … flying fish fleet sarasota

"WebMay 2, 2024 · The results from RT-qPCR and western blotting exhibited that at day 6 after incubation with M-CSF and RANKL, miR-22-3p mimic significantly decreased the mRNA … " - Ctsk western blot

Ctsk western blot

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WebApr 13, 2024 · b Western blot analysis of Ano1 protein level in Ano1 fl/fl and Ctsk-Cre;Ano1 fl/fl osteoclasts after treatment with Ctrl or HG (left). The quantification of Ano1 protein … WebWestern blotting products, and to search our catalog of over 14,000 antibodies . FIGURE 1 Overview of Western Blotting. Separation of protein mixtures by electrophoresis; transfer to a blotting membrane; and detection of target protein, which only becomes visible in the final stage as a band similar to that shown in lane 3 .

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WebTissue proteome. GENERAL INFORMATIONi. General description of the gene and the encoded protein (s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project. Gene namei. Official gene symbol, which is typically a short form of the gene name, according to HGNC. CTSK. WebOct 10, 2024 · To determine whether NFATc1 was the direct downstream target of Stat3 in osteoclasts, we examined the expression of NFATc1 in BMMs isolated from WT and …

WebAbnova Anti-CTSK Monoclonal (2F1), Catalog # H00001513-M01. Tested in Western Blot (WB), Immunohistochemistry (Paraffin) (IHC (P)) and ELISA (ELISA) applications. This … Web(B) Western blots analysis of the integrin β3, CTSK, NFATc1, and c-Fos expression induced by treatment with RANKL (50 ng/mL) for 5 days in the presence or absence of …

WebSep 5, 2024 · Western blot和RT-PCR 方法检测分化成熟脂肪细胞中I型胶原，分化转录因子过氧化物酶体增殖物激活受体γ （Peroxisome proliferators-activated receptorγ,PPARγ ）和CCAAT增强子结合蛋白α （CCAAT/enhancer-binding protein α ，C/EBPα ）蛋白和mRNA 的表达，比较各组间其 mRNA和蛋白表达的 ... WebSep 17, 2024 · Western blotting (also called immunoblotting, because an antibody is used to specifically detect its antigen) was introduced by Towbin, et al. in 1979 and is now a routine technique for protein analysis. …

WebCathepsin K antibody LS-C513306 is an APC-conjugated mouse monoclonal antibody to human Cathepsin K (CTSK). Validated for ELISA, IHC and WB.

WebTotal lysates were prepared and the expression levels of c-Src (C) and CtsK (D) were determined by Western blotting. Graphs representing densitometry analyses of c-Src (C) and CtsK (D) expression (normalized to tubulin) expressed as the mean ± SE of three independent experiments. *P < 0 and **P < 0, versus RANKL only-treated control. green line bayern 2. fremdspracheWebCathepsin K Antibody Western Blot validation (1:500 dilution) in Primary mouse osteoclasts (Cat no:11239-1-AP) FH. RICHA (Verified Customer) … green line back bayWebCathepsin K antibody LS-C375556 is an unconjugated rabbit polyclonal antibody to Cathepsin K (CTSK) (aa115-329) from human. It is reactive with human and rat. Validated for ELISA, IHC and WB. flying fish from marioWebCTSK:230111: Graphical displays and utilities; Graphs: Graphs displaying summary information of all variants in the database ... flying fish fort worthWebThe western blot (sometimes called the protein immunoblot), or western blotting, is a widely used analytical technique in molecular biology and immunogenetics to detect specific proteins in a sample of tissue homogenate or extract. Besides detecting the proteins, this technique is also utilized to visualize, distinguish, and quantify the different proteins in a … greenline beach cruiser for saleWebCTSK. General description of the gene and the encoded protein (s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project. Official gene symbol, which is typically a short form of the gene name, according to HGNC. Full gene name according to HGNC. flying fish game downloadWebMar 30, 2024 · 结果与结论：①体外实验：当帕罗西汀浓度≤5 μmol/L时对小鼠骨髓源性巨噬细胞的增殖无影响，浓度大于10 μmol/L时抑制细胞的增殖；当帕罗西汀浓度≥0.5 μmol/L时，可明显抑制破骨细胞分化的数量及形态，抑制破骨细胞分化c-Fos、Nfatc1、Ctsk、Mmp9、Acp5、Atp6v0d2的 ... flying fish flying fish